【日本生研】如何进行血清分型---How is serotyping done?

如何进行血清分型?

玻片凝集法

试管凝集法

How is serotyping done?

Slide Agglutination Method

Tube Agglutination Method

玻片凝集法

用甲醛生理盐水对机体组织进行悬浮沉降

 
在一张载玻片上滴上一滴诊断血清和一滴待检菌液

用铂丝混合2滴液体
轻摇载玻片，1分钟后观察凝集情况

有明显凝集出现即为阳性

Slide Agglutination Method

Suspend colony of organism  in formol saline

   
Add One drop of antisera +one drop of bacterial suspension to a glass slide

   
Mix two drops using a platinum wire
Rock slide and observe agglutination within 1 minute

    
Strong agglutination is considered positive

试管凝集法

将培养液在37度环境下放置6-8 小时

用甲醛生理盐水稀释培养液

滴入.05ml 的诊断血清和.5ml 的稀释待检菌液到一个 12 x 75 的试管中
(2种方法始终保持在生理盐水的环境下，以监控自动凝集)

混合，在 52度下静置1小时.

检查凝集情况 (不要摇晃)

Tube Agglutination Method

Grow culture for 6-8 hours at 37 C in broth

Dilute culture with formol saline

Add .05ml of antisera and .5ml of diluted bacterial suspension to a 12 x 75 test tube
(Always run a saline control for both methods to check for auto-agglutination)

Mix and incubate at 52 C for 1 hr.

Check for agglutination (do not shake)