【日本生研】“O”抗原的分类和测定“H” 抗原---Typing of “O” Antigen&Determination of “H” Antigen

“O”抗原的分类

玻片凝集法

先测定多价诊断血清
 A-G (DK292484) or A-S(DK292476)
O多价 (DK211231) or O1多价 (DK211248)

用特定的单价诊断血清来测定 “O” 群组

Typing of “O” Antigen

Slide Agglutination method

Determine Polyvalent First
 A-G (DK292484) or A-S(DK292476)
 Poly O (DK211231) or Poly O1 (DK211248)

Determine “O” grouping using the appropriate monovalent antisera

测定“H” 抗原

试管凝集试验

使用沙门菌属参考手册
 通常使用体细胞(O)抗原类别来测定鞭毛抗原
分类隔离
 给予特定的 “H” 多价和/或 单价诊断血清
大多数沙门氏菌是双相的
所以将会有2个 “H”抗原需要测定
相诱导

Determine “O” grouping using the appropriate monovalent antisera

Determination of “H” Antigen

Using the Salmonella Ref. Manual
 determine the flagellar antigens that are possible given the somatic (O) antigen type
Type the isolate
 with the appropriate “H” poly and/or monovalent sera
Most Salmonella are bi-phasic
so there will be 2 “H” antigens to determine
done by phase induction